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PCR analysis of the virG gene in wild-type Shigella and vaccine strain WRSS1. PCR analysis of the size of virG was carried out with primers BA114 and BA117. Products were run on an ethidium-bromide-stained 0.8% agarose gel. Lane 1, molecular size markers; lane 2, S. flexneri 2a strain 2457T; lane 3, S. flexneri 5a strain M90T-W; lane 4, S. sonnei Mosely; lane 5, WRSS1. The sizes of the wild-type virG gene and the deleted gene found in WRSS1 are shown at the right.
