TABLE 2.
Sereny test of vaccine strain WRSS1 and of WRSS1 complemented with wild-type virG gene
| Strain | Genotype | No. of eyes inoculated | No. of eyes with indicated ratinga:
|
|||
|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | |||
| S. sonnei Mosely | Wild type | 4 | 0 | 0 | 0 | 4 |
| WRSS1 | ΔvirG | 8 | 8 | 0 | 0 | 0 |
| WRSS1(pHS3188)b | virG+ | 4 | 0 | 0 | 1 | 3 |
| WRSS1(pHS3192)b | virG+ | 4 | 0 | 0 | 4 | 0 |
a
The rating system is described in the text.
b
pHS3188 contains a 7.6-kb EcoRI fragment, with the entire wild-type virG gene ligated into pBR322 (4). pHS3192 contains a 6.1-kb EcoRI-SalI fragment, with the entire virG gene from pHS3188 ligated into pBR322.