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Y2H screening of potential proteins interacting with PUB23. By Y2H assay, 272 positive clones grew on the SD/-Trp/-Leu/-His medium, and 59 ones were successfully sequenced. To verify the interaction, all the 59 clones were plated onto selective mediums (SD/-Trp/-Leu, SD/-Trp/-Leu/-His, SD/-Trp/-Leu/-His/Ade and SD/-Trp/-Leu/-His/Ade+x-α-gal).
