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. 2024 Feb 5;25(3):1930. doi: 10.3390/ijms25031930

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Kiwifruit PUB23 interacts with WT-GT1 in vitro and in vivo. (A) In vitro pull-down assay. FLAG-PUB23 and His-GT1 were expressed by cell free expression system, co-incubated, and precipitated with FLAG-tag magnetic beads or His-tag magnetic beads, respectively. The eluted proteins were then resolved by SDS-PAGE and probed with anti-FLAG or anti-His antibody. (B) In vivo co-immunoprecipitation assay. FLAG-PUB23 and GFP-GT1 were expressed in N. benthamiana and purified with GFP-tag magnetic beads or FLAG-tag beads. Components were immunoblotted with anti-GFP or anti-FLAG antibody.