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. 2024 Jan 31;29(3):669. doi: 10.3390/molecules29030669

Figure 9.

Figure 9

Detoxification of mixed dyes (four- and seven-dye mixture) with the treatment with the crude laccase from P. eryngii. (A) The toxicity of untreated and decolorized NC (azo) + RB5 (azo) + RB4 (anthraquinone) + RBBR (anthraquinone) to wheat seedling shoot growth. Control: acetic acid–sodium acetate buffer (pH = 5.0) added (not adding dye). 100-0: 100 mg/L untreated dye mixture (the concentration of each dye was 100 mg/L). 100-1: 100 mg/L decolorized dye mixture (the concentration of each dye was 100 mg/L). 200-0: 200 mg/L untreated dye mixture (the concentration of each dye was 200 mg/L). 200-1: 200 mg/L decolorized dye mixture (the concentration of each dye was 200 mg/L). 600-0: 600 mg/L untreated dye mixture (the concentration of each dye was 600 mg/L). 600-1: 600 mg/L decolorized dye mixture (the concentration of each dye was 600 mg/L). p < 0.01 indicates a highly significant difference (**). p < 0.05 indicates a significant difference (*). (B) The toxicity of untreated and decolorized NC (azo) + RBBR (anthraquinone) + BB (triphenylmethane) + IC (indigo) to wheat seedling shoot growth. Control: acetic acid–sodium acetate buffer (pH = 5.0) added (not adding dye). 400-0: 400 mg/L untreated dye mixture (the concentration of each dye was 400 mg/L). 400-1: 400 mg/L decolorized dye mixture (the concentration of each dye was 400 mg/L). p < 0.01 indicates a highly significant difference (**). (C) The toxicity of untreated and decolorized NC (azo) + RBBR (anthraquinone) + BB (triphenylmethane) + IC (indigo) to wheat seedling root growth. Control: acetic acid–sodium acetate buffer (pH = 5.0) added (not adding dye). 200-0: 200 mg/L untreated dye mixture (the concentration of each dye was 200 mg/L). 200-1: 200 mg/L decolorized dye mixture (the concentration of each dye was 200 mg/L). 400-0: 400 mg/L untreated dye mixture (the concentration of each dye was 400 mg/L). 400-1: 400 mg/L decolorized dye mixture (the concentration of each dye was 400 mg/L). 800-0: 800 mg/L untreated dye mixture (the concentration of each dye was 800 mg/L). 800-1: 800 mg/L decolorized dye mixture (the concentration of each dye was 800 mg/L). p < 0.01 indicates a highly significant difference (**). p < 0.05 indicates a significant difference (*). (D) The toxicity of untreated and decolorized NC (azo) + RB5 (azo) + RBBR (anthraquinone) + RB4 (anthraquinone) + MG (triphenylmethane) + BB (triphenylmethane) + IC (indigo) to wheat seedling shoot growth. Control: acetic acid–sodium acetate buffer (pH = 5.0) added (not adding dye). 200-0: 200 mg/L untreated seven-dye mixture (the concentration of each dye was 200 mg/L). 200-1: 200 mg/L decolorized seven-dye mixture (the concentration of each dye was 200 mg/L). 400-0: 400 mg/L untreated seven-dye mixture (the concentration of each dye was 400 mg/L). 400-1: 400 mg/L decolorized seven-dye mixture (the concentration of each dye was 400 mg/L). p < 0.01 indicates a highly significant difference (**). (E) The toxicity of untreated and decolorized NC (azo) + RB5 (azo) + RBBR (anthraquinone) + RB4 (anthraquinone) + MG (triphenylmethane) + BB (triphenylmethane) + IC (indigo) to rice seedling shoot growth. Control: acetic acid–sodium acetate buffer (pH = 5.0) added (not adding dye). 100-0: 100 mg/L untreated seven-dye mixture (the concentration of each dye was 100 mg/L). 100-1: 100 mg/L decolorized seven-dye mixture (the concentration of each dye was 100 mg/L). p < 0.01 indicates a highly significant difference (**). NC: New Coccine; RB5: Reactive Black 5; RBBR: Remazol Brilliant Blue R; RB4: Reactive Blue 4; MG: Methyl Green; BB: Bromophenol Blue; IC: Indigo Carmine.