Table 1.

Hydrolytic activity values of free lipase and immobilized biocatalysts in the studied variations.

Biocatalysts		Hydrolytic Activity (U g−1)	Specific Activity (U mg−1)	Relative Activity (%)
BCL-free		42.62	-	100
HNF-BCL pH 7.4	10 mM	171.81	219.91	403.11
HNF-BCL pH 6	25 mM	217.34	257.33	509.96
HNF-BCL pH 7	25 mM	415.38	432.00	974.62
HNF-BCL pH 7.4	25 mM	518.00	538.07	1215.38
HNF-BCL pH 8	25 mM	182.32	188.01	427.77
HNF-BCL pH 6	50 mM	281.67	301.47	660.88
HNF-BCL pH 7	50 mM	398.42	432.39	934.81
HNF-BCL pH 7.4	50 mM	521.35	541.88	1223.26
HNF-BCL pH 8	50 mM	263.64	278.17	618.59
HNF-BCL pH 6	100 mM	362.19	410.30	849.82
HNF-BCL pH 7	100 mM	540.11	623.85	1267.27
HNF-BCL pH 7.4	100 mM	888.43	884.96	2084.96
HNF-BCL pH 8	100 mM	178.63	330.46	419.12