Table 3.
Hook effect and dilution linearity.
| Nominal Conc. (μg/mL) |
Dilution Factor |
Measured Conc. (μg/mL) |
Dilution Factor |
Measured Conc. (μg/mL) |
Mean Conc. (μg/mL) |
CV % | Bias % |
|---|---|---|---|---|---|---|---|
| 1000 | 1 | AQL | 200 | 924 | 914 | 4.7 | −8.6 |
| AQL | 888 | ||||||
| AQL | 936 | ||||||
| AQL | 965 | ||||||
| AQL | 855 | ||||||
| 800 | 1 | AQL | 100 | 656 | 658 | 5.8 | −17.8 |
| AQL | 657 | ||||||
| AQL | 681 | ||||||
| AQL | 598 | ||||||
| AQL | 698 | ||||||
| 500 | 1 | AQL | 50 | 503 | 470 | 7.1 | −6.0 |
| AQL | 510 | ||||||
| AQL | 442 | ||||||
| AQL | 442 | ||||||
| AQL | 455 | ||||||
| 250 | 1 | AQL | 20 | 214 | 222 | 2.8 | −11.2 |
| AQL | 218 | ||||||
| AQL | 223 | ||||||
| AQL | 225 | ||||||
| AQL | 230 | ||||||
| 125 | 1 | AQL | 10 | 92.3 | 100 | 8.9 | −20.0 |
| AQL | 91.2 | ||||||
| AQL | 99.9 | ||||||
| AQL | 106 | ||||||
| AQL | 112 |