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. 2024 Jan 30;13(3):408. doi: 10.3390/plants13030408

Figure 4.

Figure 4

Effect of prolonged incubation with NPA on seed germination. (a) Scheme of the experimental protocol. Seeds were incubated for 2 h in darkness and then irradiated with an FRp. Seeds were then incubated for 3 days at 5 °C and irradiated with an Rp. Germination was counted after 3 days at 25 °C. The blue bars represent the incubation period in water and the green bars represent the incubation period in NPA 200 μM. (b) Germination percentage of Col-0 and auxin carriers mutant seeds in response to an Rp. The incubation medium was supplemented with NPA 200 μM for variable periods after seed imbibition (0 h, 72 h, 77 h, 96 h, 102 h, 120 h, and 144 h), keeping a control in water (0 h) and a control in NPA 200 μM (144 h). Each point represents the mean ± SE (n ≥ 6). Different letters denote significant differences among means at the same time point (p < 0.05 by ANOVA followed by Tukey posttest). Rp: red pulse.