TABLE 2.
Number of Neo gene copies of M12-neo-1 cells after lysis by CTL and DNase treatmenta
| Time (h) | Neo gene copy no. with the following CTL/DNase treatment:
|
% Specific 51Cr release | |||
|---|---|---|---|---|---|
| −/− | −/+ | +/− | +/+ | ||
| 0 | 195 ± 30 | 180 ± 17 | ND | ND | ND |
| 1 | ND | ND | 199 ± 24 | 64 ± 3 | 48 |
| 2 | ND | ND | 210 ± 36 | 26 ± 12 | 67 |
| 4 | ND | ND | 188 ± 35 | 4 ± 2 | 78 |
a
51Cr-labeled M12-neo-1 cells (104) were incubated or not incubated with alloreactive CTL cells (105) for 1 to 4 h, and treated or not treated with DNase. The number of Neo gene copies was determined by QC-PCR and is expressed as the mean copy number per template DNA, corresponding to 300 input M12-neo-1 cells ± 1 SD. The percent specific 51Cr release was determined by a standard method in the same experiment. Similar results were obtained in three independent experiments. ND, not done.