TABLE 3.
Effect of T. gondii-specific CTL on the viability of intracellular T. gondiia
| CTL | Infection of target cells with T. gondii | No. of copies of the following gene with the indicated DNase treatment:
|
|||
|---|---|---|---|---|---|
| Neo
|
SAG-1
|
||||
| − | + | − | + | ||
| − | − | 136 ± 40 | 118 ± 30 | ND | ND |
| − | + | 148 ± 32 | 144 ± 20 | 110 ± 14 | 140 ± 52 |
| + | − | 124 ± 7 | 122 ± 25 | ND | ND |
| + | + | 137 ± 27 | 60 ± 19 | 102 ± 8 | 110 ± 9 |
a
M12-neo-1 cells (104) which were infected (+) or not infected (−) with T. gondii were incubated or not incubated with CTL specific for T. gondii for 4 h at an effector/target cell ratio of 50:1 prior to DNase treatment. The numbers of Neo and SAG-1 gene copies were determined by QC-PCR as described in Materials and Methods and are expressed as the mean copy number for triplicate experiments ± 1 SD. Fifty-one percent of the target cells were infected with T. gondii, as determined by light microscopy. ND, not done.