Figure 4.

M. orbicularis’ fruits’ ethanolic extracts show potent anti-hemolytic activity. (A) Hemolytic activity assay. Erythrocytes were treated with different concentrations (10, 20, 40, 50, and 100 μg/mL) of ethanolic extract of fruits of M. orbicularis. Bar graphs represent mean % hemolysis (compared to vehicle-control-treated RBCs) of 3 independent experiments. (B) Erythrocytes were pretreated with different (40, 50, and 100 μg/mL) concentrations of ethanolic extracts from the fruits of M. orbicularis. H₂O₂ was then added to induce hemolysis. Data represent % inhibition of hemolysis compared to control-(ctr) vehicle-treated cells. Data are displayed as the mean ± SEM of three independent experiments; **** denotes p < 0.0001.