RFLP patterns of the UHB element among B. burgdorferi sensu lato complex isolates. HaeIII-digested DNA was transferred onto a Hybond N membrane and hybridized with the uhb(+) oligonucleotide under conditions described in the text. The isolates analyzed are indicated above the lanes, and molecular size standards (in kilobases) are indicated on the right. Isolates N34, G25, VSBP, Pbi, FRG, B4-91, and B487 are B. garinii; VS116 is B. valaisiana; ECM1 and UMO1 are B. afzelii; IKA2 and HO14 are B. japonica; 21038 is B. andersonii; and N40, CA13, CA3, CA8, IP89, CA9, LP3, VS307, VS134, and T2 are B. burgdorferi.