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. 2024 Jan 28;13(3):389. doi: 10.3390/plants13030389

Figure 1.

Figure 1

Agarose gel electrophoresis (AGE) of RNA isolated from needle (ND), phloem (PH), and root (RT) tissues using different RNA isolation methods, i.e., RNeasy® Plant Mini Kit, Spectrum Plant Total RNA Kit, standard TRIzol, TRIzol-column hybrid, and the modified CTAB protocol (numbers after tissue name, viz. 1–2 indicate biological controls). gDNA shows genomic contamination of the sample isolate. The RNA yield and absorbance ratios are mentioned in Table 1. To facilitate comparison, an equal volume of RNA (4 µL) was loaded due to the unavailability of sufficient RNA concentration in certain samples to be loaded in equal amounts (50 or 100 ng).