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. 2024 Feb 8;20(1):2309693. doi: 10.1080/21645515.2024.2309693

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 2. — Development of ERmut+ mouse mammary tumor model. (a) MM3MG cell lines expressing Y537N or Y537S doxycycline inducible ESR1 mutants were assessed at 24 hours post-dox/estrogen stimulation for the indicated pathway (n = 3/group). (b) QRT-PCR assessment of ESR1 expression in MM3MG lines. (c) Indicated MM3MG cells were implanted into BALB/c mice and allowed to grow, measured bi-weekly (n = 5 mice/group). (d) Indicated MM3MG cells were implanted into BALB/c mice and allowed to grow, measured bi-weekly (n = 5 mice/group).