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The per plate column corresponds to 110 wells per plate to account for reagent loss. The Exo reagents listed are part of the TaqMan Exogenous Internal Positive Control reagent kit (Applied Biosystems, Waltham MA).
| Reagent | Volume per Well (μL) | Volume per Plate (μL) |
|---|---|---|
| TaqPath TM Master Mix | 5.0 | 550.0 |
| Primer-Probe-Water Mix | 4.0 | 440.0 |
| 50X Exo IPC DNA | 0.4 | 44.0 |
| 10X IPC Exo mix | 2.0 | 220.0 |
| Water | 3.6 | 396.0 |
