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. 2024 Feb 9;19(2):e0298087. doi: 10.1371/journal.pone.0298087

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© 2024 Nguyen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — The cLAMP specificity of the design (left panel) and reference (right panel) primer set. The plasmid DNA (pDNA) containing 18S rRNA genes from both the P. falciparum S-type (PfS) and A-type (PfA) were utilized to confirm primer specificity. A-type 18S rRNA genes from other Plasmodium species were also employed for confirmation: P. vivax (PvA), P. knowlesi (PkA), P. malariae (PmA), and P. ovale (PoA). (a) The results indicated the specificity of PCR with the outer primer set (F3-B3c) for their corresponding fragments. PfA, with the designed primer, exhibited an expected size of 229 bp, while PfS, with the reference primer, showed an expected size of 220 bp. Lane M represents the DNA ladder mix. (b) The cLAMP results were visualized through 2% agarose gel electrophoresis. (c) The cLAMP results corresponded to (b) and were confirmed through naked eye direct observation. Yellow/orange coloration indicated a positive, whereas pink indicated a negative.