Fig 3. Limit of detection based on plasmid DNA.

(a) PCR detection using the outer primer set with serially diluted plasmid DNA. Ten microliters of the PCR products were visualized on a 2% agarose gel. (b) One microliter of the endpoint products from the cLAMP reaction was observed on a 2% agarose gel. (c) cLAMP results were interpreted by the naked eye; yellow/orange indicates a positive result, whereas pink indicates a negative result. The lane contained P. vivax gDNA (P. vivax) and DDW, which were used as negative controls.