Fig 4. Limit of detection test based on parasite count.

(a) PCR detection using the outer primer set with serially diluted parasites genomic DNA (parasites/μL; p/μL). Ten microliters of the amplicon were visualized on a 2% agarose gel. (b) One microliter of the cLAMP product was visualized on a 2% agarose gel. Negative control consisting of P. vivax gDNA (P. vivax) and DDW were used to confirm specificity. (c) The cLAMP results corresponding to (b) were visualized with the naked eye; yellow indicated a positive, whereas pink indicated a negative.