Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | Caenorhabditis Genetics Centre | Wormbase, E. coli OP50 | WBStrain00041969 | Other names: CBb1 |
| Recombinant DNA reagent | Source Bioscience | Vidal and Ahringer RNAi Libraries in HT115 (D3) E. coli | RNAi Library | |
| Chemical compound, drug | L-methionine | Fisher Scientific | Cat#: AC166160025 CAS: 63-68-3 | |
| Chemical compound, drug | L-Cystathionine | Sigma | Cat#: C7505-10MG | |
| Chemical compound, drug | L-Methionine | Sigma | Cat#: M-9625 | |
| Chemical compound, drug | L-Serine | Sigma | Cat#: S-4500 | |
| Chemical compound, drug | Choline Chloride | Millipore Sigma | Cat#: C7017-5G, CAS:67-48-1 | |
| Chemical compound, drug | Sodium Chloride (NaCl) | Fisher Bioreagents | Cat#: BP358-1 CAS:7647-14-5 | |
| Chemical compound, drug | β-meracaptoethanol | Fisher Scientific | Cat#: AC125470100 CAS: 60-24-2 | |
| Chemical compound, drug | Trizol | ThermoFisher Scientific | Cat#: 15596018 | |
| Chemical compound, drug | PowerUp SYBR Green Master Mix | Applied Biosystems | Cat#: A25741 | |
| Chemical compound, drug | Agarose | Fisher Bioreagents | Cat#: BP160-500, CAS: 9012-36-6 | |
| Chemical compound, drug | Pronase | Sigma- Aldrich | SKU# 10165921001 | |
| Chemical compound, drug | DMSO (Dimethyl Sulfoxide) | ThermoFisher Scientific | Cat#: 85190, Cas:67-68-5 | |
| Chemical compound, drug | Water, Molecular Grade, Sterile, DEPC Free | Fisher Scientific | CAS: 7732-18-5 Cat#: R91450001G, | |
| Chemical compound, drug | NCT502 | MedChemExpress (MCE) | Cat#: HY-117240 | |
| Chemical compound, drug | Polybead polystyrene | Polysciences | Cat#08691–10 | |
| Commercial assay or kit | ATP Assay Kit (Colorometric/ Fluorometric) | Abcam | Ab83355 | |
| Commercial assay or kit | Pyrophosphate Assay Kit (Fluorometric) | Abcam | Ab112155 | |
| Commercial assay or kit | Pyruvate Kinase (PK) Assay Kit (Colorimetric) | Abcam | Ab83432 | |
| Commercial assay or kit | BCA Protein Quantification Kit | Abcam | Ab102536 | |
| Commercial assay or kit | RNAeasy columns | QIAGEN | Cat#74034 | |
| Commercial assay or kit | Direct-zol RNA Miniprep Plus Kit | Zymo Research | Cat# R2070 | |
| Strain, strain background (C. elegans) | SK4005 | Taub et. al. | WT (zdis5 pmec-4::GFP) | |
| Strain, strain background (C. elegans) | NA | Taub et. al. | ogt-1(ok1474)_zdis-5 pmec-4::GFP | |
| Strain, strain background (C. elegans) | OG1135 | This study | Ogt-1 (OG1135)_TU3568_ pmec-4::GFP | |
| Strain, strain background (C. elegans) | RB1342 | Taub et. al. | ogt-1(ok1474) | |
| Strain, strain background (C. elegans) | NA | Taub et. al. | TU3568 (sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71[(pCFJ90) pmyo-2::mCherry +pmec-18::sid-1]) | |
| Strain, strain background (C. elegans) | NA | Taub et. al. | ogt-1(ok1474)_TU3568 | |
| Strain, strain background (C. elegans) | RB2240 | CGC | sams-1(ok3033) | |
| Strain, strain background (C. elegans) | NA | in this study | sams-1_zdis-5 | |
| Strain, strain background (C. elegans) | NA | in this study | ogt-1;sams-1_zdis-5 | |
| Strain, strain background (C. elegans) | RB755 | CGC | metr-1(R03D7.1(ok521)) | |
| Strain, strain background (C. elegans) | NA | in this study | metr-1_zdis-5 | |
| Strain, strain background (C. elegans) | NA | in this study | ogt-1;metr-1_zdis-5 | |
| Strain, strain background (C. elegans) | VC1011 | CGC | acdh-1(ok1489) | |
| Strain, strain background (C. elegans) | NA | in this study | acdh-1_zdis-5 | |
| Strain, strain background (C. elegans) | NA | in this study | ogt-1;acdh-1_zdis-5 | |
| Strain, strain background (C. elegans) | RB512 | CGC | mce-1(D2030.5(ok243)) | |
| Strain, strain background (C. elegans) | NA | in this study | mce-1_zdis-5 | |
| Strain, strain background (C. elegans) | NA | in this study | mce-1_TU3568_zdis-5 | |
| Strain, strain background (C. elegans) | NA | in this study | ogt-1;mce-1_TU3568_zdis-5 | |
| Strain, strain background (C. elegans) | CG122 | Taub et. al. | ogt-1;akt-1(mg144) | |
| Strain, strain background (C. elegans) | CG125 | Taub et. al. | ogt-1;akt-1(ok525) | |
| Strain, strain background (C. elegans) | MS2495 | Soto and Rivera et. al. | irIs158 (normal ATP sensor, CAmA) | |
| Strain, strain background (C. elegans) | NA | In this study | ogt-1; CAmA | |
| Sequence-based reagent | folr-1_F | C17G1.1 | RT-qPCR | GGCTTCCATTGCCGTCATAA |
| Sequence-based reagent | folr-1_R | C17G1.1 | RT-qPCR | GCTAACCACTGGCTCACGAT |
| Sequence-based reagent | metr-1_F | R03D7.1 | RT-qPCR | CCCGAATCGCAGTTATCCGA |
| Sequence-based reagent | metr-1_R | R03D7.1 | RT-qPCR | GAAGCAGCTGGGAGGAATGA |
| Sequence-based reagent | sams-1_F | C49F5.1 | RT-qPCR | CACTCACCGACGAAGAGCTT |
| Sequence-based reagent | sams-1_R | C49F5.1 | RT-qPCR | GTGACCGAAGTGACCGTTCT |
| Sequence-based reagent | dmat-1_F | C18A3.1 | RT-qPCR | ATTGCCGATCCACCATGGTT |
| Sequence-based reagent | dmat-1_R | C18A3.1 | RT-qPCR | CCGATTTGTGATCCAGAAAGCA |
| Sequence-based reagent | nmad-1_F | F09F7.7 | RT-qPCR | GCACAGTCACAAAGTGGTCG |
| Sequence-based reagent | nmad-1_R | F09F7.7 | RT-qPCR | CGTACTCTGGCATTCCGACA |
| Sequence-based reagent | cth-1_F | F22B8.6 | RT-qPCR | TCTGATATTATTATGGGAGCCGC |
| Sequence-based reagent | cth-1_R | F22B8.6 | RT-qPCR | TGCAGTCATGAGCTTCAAGGA |
| Sequence-based reagent | cth-2_F | ZK1127.10 | RT-qPCR | TTGGAGCGGATGTTGTCGTT |
| Sequence-based reagent | cth-2_R | ZK1127.10 | RT-qPCR | AGTGAGCTCTCATTCTGATGTGA |
| Sequence-based reagent | pcca-1_F | F27D9.5 | RT-qPCR | AAATGGGAGAACAGGCCGTT |
| Sequence-based reagent | pcca-1_R | F27D9.5 | RT-qPCR | TGGGTGATTGGAAGTGGGTG |
| Sequence-based reagent | pccb-1_F | F52E4.1 | RT-qPCR | AAAGTTTGCTGCTGGATGCC |
| Sequence-based reagent | pccb-1_R | F52E4.1 | RT-qPCR | AATCTTTGGAACGGTGGCCT |
| Sequence-based reagent | mce-1_F | D2030.5 | RT-qPCR | TGTCCACAAGAACCATGGCT |
| Sequence-based reagent | mce-1_R | D2030.5 | RT-qPCR | CGCCGAATGGATGAAGAAGC |
| Sequence-based reagent | mmcm-1_F | ZK1058.1 | RT-qPCR | CAATGTTGCCGATCCTTGGG |
| Sequence-based reagent | mmcm-1_R | ZK1058.1 | RT-qPCR | TCCAACAATCACATCTTTTCCAGC |
| Sequence-based reagent | acdh-1_F | C55B7.4 | RT-qPCR | TCCGAGCTTCATCCACTTGT |
| Sequence-based reagent | acdh-1_R | C55B7.4 | RT-qPCR | CTGACCGAACTGTTCTCTCTGT |
| Sequence-based reagent | ech-6_F | T05G5.6 | RT-qPCR | AGGTGGAAACGAGTTGGCAA |
| Sequence-based reagent | ech-6_R | T05G5.6 | RT-qPCR | GCTCACAATACCGTGCTCCT |
| Sequence-based reagent | hach-1_F | F09F7.4 | RT-qPCR | AGTCATCAGATCGTTCGAGCC |
| Sequence-based reagent | hach-1_R | F09F7.4 | RT-qPCR | TCGGTGATTTGTCGGTGAGT |
| Sequence-based reagent | hphd-1_F | Y38F1A.6 | RT-qPCR | CAAAGATCTCCACGCCCTGA |
| Sequence-based reagent | hphd-1_R | Y38F1A.6 | RT-qPCR | GGAGAGTCCGTGGCAAAGAT |
| Sequence-based reagent | alh-8_F | F13D12.4 | RT-qPCR | GGGAGCTCAGGTTCCACTTG |
| Sequence-based reagent | alh-8_R | F13D12.4 | RT-qPCR | TGAAGATGGCCGTTCCGTTT |
| Sequence-based reagent | act-1_F | T04C12.6 | RT-qPCR | TCGGTATGGGACAGAAGGAC |
| Sequence-based reagent | act-1_R | T04C12.6 | RT-qPCR | CATCCCAGTTGGTGACGATA |