Figure 5.

Stabilization of different homotrimeric proteins via bicyclization

(A) Characterization of l4₃Ta₂ (Ip1913, PDB: 3fnj).

(B) Characterization of b4₃Ta₂ (BH3489, PDB: 1vmf).

(C) Characterization of a4₃Ta₂ (Au4130, PDB: 3c6v).

(D) Characterization of e4₃Ta₂ (ECHD, PDB: 5c9g). Reported crystal structures and cysteine variation sites with average distances (d_avg) between Cα atoms of cross-linking residues are shown. Calculated and found molecular weights (MWs) and CD-derived thermal denaturation curves of unmodified (black) and corresponding cross-linked trimers (orange) are provided, including the resulting difference in melting temperatures (ΔT_m). T_m values (Table S12) represent the maximum of the first derivative (corresponding HT curves and CD spectra are available in Figure S18).