Skip to main content
. Author manuscript; available in PMC: 2024 Jun 5.
Published in final edited form as: Nat Cardiovasc Res. 2023 Dec 5;2(12):1277–1290. doi: 10.1038/s44161-023-00388-7

Fig. 3 |. Fatty acid oxidation is required for emergency hematopoiesis after myocardial infarction.

Fig. 3 |

A, Quantification of blood leukocytes, monocytes and neutrophils (PMN) by flow cytometry in Cpt1afl/fl controls and Vav1Cre/+Cpt1Afl/fl mice, both 3 days after MI (n=6 Cpt1afl/fl, n= 7 Vav1Cre/+Cpt1Afl/fl, two-tailed Welch’s t test, three independent experiments). B, Quantification of bone marrow LSK, CMP and GMP by flow cytometry in Cpt1afl/fl controls and Vav1Cre/+Cpt1Afl/fl mice 3 days after MI (n=7 Cpt1afl/fl, n=10 Vav1Cre/+Cpt1Afl/fl, two-tailed Welch’s t test, three independent experiments). C, Colony forming unit (CFU) assay of bone marrow cells from Cpt1afl/fl controls and Vav1Cre/+Cpt1Afl/fl mice 3 days after MI (n=10 Cpt1afl/fl, n=10 Vav1Cre/+Cpt1Afl/fl, two-tailed Welch’s t test). D, Proliferation quantification of LSK, CMP and GMP in the femurs of Cpt1afl/fl controls and Vav1Cre/+Cpt1Afl/fl mice 3 days after MI (n=7 Cpt1afl/fl, n=11 Vav1Cre/+Cpt1Afl/fl, two-tailed Welch’s t test, three independent experiments). E, Flow plots and F, quantification of monocytes and neutrophils in the ischemic myocardium of Cpt1afl/fl controls and Vav1Cre/+Cpt1afl/fl mice on day 3 after MI (n=12 Cpt1afl/fl, n=11 Vav1Cre/+Cpt1Afl/fl, two-tailed Welch’s t test, four independent experiments). Data are displayed as mean±SEM.