Video 2.
Full rings of APOE surrounding LDs contacting the ER. Airyscan live-cell imaging of TRAE3-H cells transfected with the ER lumen marker TagBFP2-KDEL (cyan) and APOE3-mEm (yellow), stained for LDs (magenta) with BODIPY 665/676, and treated with 400 µM OA for 4 h. The video shows full rings of APOE on the surface of LDs and at ER-LD contact sites. Scale bar: 1 µm. Corresponds to the images shown in Fig. 3 A. Cells were imaged every 4.9 s for 29 frames. The video plays at 10 frames per second.