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FRAP of LD-associated APOE after an OA pulse-chase. Confocal FRAP movies of primary astrocytes transfected with APOE3-mEm (green), stained for LDs with BODIPY 665/676 (magenta), and treated with 200 µM OA for 4 h followed by a 2-h chase in complete media. LD-associated APOE recovers very slowly when bleached after an OA pulse-chase. Scale bar: 1 µm. Corresponds to images shown in Fig. 3 C. Cells were imaged every second for 96 frames. The video plays at 5 frames per second.
