Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Feb 9;15:1229. doi: 10.1038/s41467-024-45201-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Reintroduction of 129/Sv genes by backcross of EGT-315 B6 (F9-F11) (mean = 0.79, SD = 2.23, n = 40) with 129/Sv increases ERV-GFP expression in EGT-315 B6 x 129/Sv (F1) mice (mean = 8.91, SD = 14.0, n = 32). C57BL/6 (mean = 0, SD = 0, n = 9) and (129/Sv x C57BL/6) F1 wt littermates (mean = 0.063, SD = 0.122, n = 9). Red bars show mean. Flowcytometry of blood leukocytes at 3 weeks of age. ***p = 0.0008. Ordinary one-way ANOVA Tukey´s multiple comparsion test. b Epigenetic DNA methylation status of a 549 bp DNA fragment of ERV-GFP in GFP positive (+) vs GFP negative (-) cells isolated from spleen cells of EGT-315 mice. DNA was digested with methylation sensitive HpaII (recognition site CCGG) and normalized with Plcg2 specific PCR w/o HpaII recognition site. ES-cells are ERV-GFP containing but GFP-expression negative and ERV-GFP infected WEHI-231 cells strongly express GFP. From individual mice sorted GFP⁺ cells (mean = 4.91, SD = 4.32, n = 9), sorted GFP^- cells (mean = 3.3, SD = 1.9, n = 15), WEHI-231 GFP⁺ (mean = 0.37, SD = 0.12, n = 4), ES cells GFP^- (mean = 26.9, SD = 8.3, n = 4). Sorted GFP⁺ cells vs Sorted GFP^- cells ^nsP = 0.752. Sorted GFP⁺ cells vs ES cells GFP^- ****P < 0.0001. Sorted GFP^- cells vs ES cells GFP^- ****P < 0.0001. WEHI-231 GFP⁺ vs ES cells GFP^-****P < 0.0001. Ordinary one-way ANOVA Tukey´s multiple comparsion test. Summary of 3 experiments. c Q-PCR of RNA expression of the GFP and Env region (549 bp) of ERV-GFP transcribed in spleen of EGT-315 mice (left panel). Sequence analysis of RNA expression analysis by RT-PCR-transcripts of EGT-315 mice with and without human Apobec3 (hA3Tg). Each dot depicts the mean number of mutations of 4–8 clones sequenced per individual mouse of the different genotypes (right panel). EGT-315 B6 (green bar, mean = 1, SD = 1.9, n = 4), EGT-315 Tlr3^−/−Tlr7^−/−Tlr9^−/− (blue bar, mean = 0.06, SD = 0.09, n = 5), EGT-315 Tlr3^−/−Tlr7^−/−Tlr9^−/− hA3 (purple bar, mean = 7.3, SD = 6.0, n = 5). Statistics of mutations EGT-315 Tlr3^−/−Tlr7^−/−Tlr9^−/− versus EGT-315 Tlr3^−/−Tlr7^−/−Tlr9^−/− hA3 *p = 0.028 by Ordinary one-way ANOVA Tukey´s multiple comparsion test. Source data are provided as a Source Data file.