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. 2024 Feb 9;14:3335. doi: 10.1038/s41598-024-53976-3

Figure 2.

Figure 2

Ferroptosis is involved in LPS-induced HUVEC inflammation. (A) The cell viability 24 h after LPS (0, 0.5, 1, 2, 4, and 8 μg/mL) treatment was assayed by CCK-8; *P < 0.05, **P < 0.01, ***P < 0.001 versus 0 μg/mL group. (B) Western blot was used to detect the expression levels of inflammation-related proteins; **P < 0.01, ***P < 0.001 versus 0 h group. (C) The intracellular iron ion, GSH, and MDA levels were measured after 2 μg/mL LPS treatment at different times (0 h,1 h,3 h,6 h,9 h,12 h,24 h); **P < 0.01, ***P < 0.001 versus the 0 h group. (D) The DCFH-DA probes were performed for intracellular ROS levels (scale bar, 200 μm) after 2 μg/mL LPS treatment for different times (0 h,1 h,3 h,6 h,9 h). (E) Western blot was used to detect the expression levels of ferroptosis-related proteins after 2 μg/mL LPS treatment for different times (0 h,1 h,3 h,6 h,9 h,12 h,24 h); *P < 0.05, ***P < 0.001 versus 0 h group.