Skip to main content
. 1998 Oct;66(10):4733–4741. doi: 10.1128/iai.66.10.4733-4741.1998

FIG. 3.

FIG. 3

Production of an hgpB mutant in H. influenzae HI689. (A) hgpB locus and targeting construct. A 1,536-bp fragment from hgpB was deleted and replaced with the tetracycline resistance (tet) marker. The targeting construct was used to transform H. influenzae to tetracycline resistance. Southern analyses of H. influenzae HI689 and mutant derivatives were performed with the 666-bp BclI fragment from hgpB (B), the 870-bp BclI fragment from hgpB (C), and the tetracycline resistance cassette (D) as probes. Lanes: 1, labeled λ HindIII digest; 2, H. influenzae HI689 chromosomal DNA BglII/EcoRI digest; 3, H. influenzae HI689 hgpAΔBglII chromosomal DNA BglII/EcoRI digest; 4, H. influenzae HI689 hgpBΔBclI chromosomal DNA BglII/EcoRI digest; lanes 5, H. influenzae HI689 hgpAΔBglII/hgpBΔBclI chromosomal DNA BglII/EcoRI digest.