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Southern analyses of H. influenzae HI689 and mutant derivatives probed using a 2,557-bp BglII fragment of hgpA (A) and the ribostamycin resistance marker TSTE (B) as probes. Lanes: 1, labeled λ HindIII digest; 2, H. influenzae HI689 chromosomal DNA BglII/EcoRI digest; 3, H. influenzae HI689 hgpAΔBglII chromosomal DNA BglII/EcoRI digest; 4, H. influenzae HI689 hgpBΔBclI chromosomal DNA BglII/EcoRI digest; 5, H. influenzae HI689 hgpAΔBglII/hgpBΔBclI chromosomal DNA BglII/EcoRI digest.
