TABLE 2.
Antibodies used in this study.
| Protein | Product number/clone name | Host species | Application | Product details |
|---|---|---|---|---|
| Primary antibodies | ||||
| DYKDDDDK (FLAG) | D6W5B | Rabbit | Western blot (1:1000) Immunofluorescence (1:200) | Cell signalling |
| SARS‐CoV‐2 Spike Receptor Binding Domain (sRBD) | S‐Ab401.1 | Human | Immunofluorescence (1:100) | ALSTEM |
| SARS‐CoV‐2 Spike Receptor Binding Domain (sRBD) | P06DHu | Human | Immunofluorescence (1:100) | ThermoFisher |
| LAMP1 | H4A3 | Mouse | Immunofluorescence (1:200) | Developmental Studies Hybridoma Bank |
| CD63 | H5C6 | Mouse | Western blot (1:1000), Immunofluorescence (1:200) | BioLegend |
| CD9 | Ab92726 | Rabbit | Western blot (1:1000) | Abcam |
| GAPDH | 2118S | Rabbit | Western blot (1:2000) | Cell signalling |
| Syntenin | Ab133267 | Rabbit | Western blot (1:1000) | Abcam |
| Tsg101 | 4A10 | Mouse | Western blot (1:1000) | GeneTex |
| ALIX | 3A9 | Mouse | Western blot (1:1000) | GeneTex |
| Calnexin | Ab133615 | Rabbit | Western blot (1:1000) | Abcam |
| FLAG (APC) | 637307 | Rat IgG2aλ | Flow cytometry (1:1000) | BioLegend |
| CD63 (FITC) | 353005 | Mouse IgG1κ | Flow cytometry (1:1000) | BioLegend |
| IgG2aλ, isotype control, Rat APC | 402305 | Rat IgG2aλ | Flow cytometry (1:1000) | BioLegend |
| IgG1κ, isotype control, Mouse, FITC | 400107 | Mouse IgG1κ | Flow cytometry (1:1000) | BioLegend |
| Secondary antibodies | ||||
| Anti‐rabbit IgG Alexa Fluor 555 | A‐21428 | Goat | Immunofluorescence (1:200) | ThermoFisher |
| Anti‐mouse IgG Alexa Fluor 488 | A‐11001 | Goat | Immunofluorescence (1:200) | ThermoFisher |
| Anti‐mouse IgG Alexa Fluor 555 | A‐21422 | Goat | Immunofluorescence (1:200) | ThermoFisher |
| Anti‐mouse IgG Alexa Fluor 647 | A‐21235 | Goat | Immunofluorescence (1:200) | ThermoFisher |
| Anti‐human IgG Alexa 488 | A‐11013 | Goat | Immunofluorescence (1:200) | ThermoFisher |
| Anti‐rabbit IRDye 680 | 926‐68071 | Goat | Western blot (1:10,000) | Li‐Cor |
| Anti‐mouse IRDye 800 | 926‐32210 | Goat | Western blot (1:10,000) | Li‐Cor |
| Anti‐human IRDye 680 | 926‐68078 | Goat | Western blot (1:10,000) | Li‐Cor |