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. Author manuscript; available in PMC: 2024 Feb 10.
Published in final edited form as: Cell Rep. 2023 Oct 5;42(10):113190. doi: 10.1016/j.celrep.2023.113190

Figure 1. Binding of selected polyreactive mouse IgA to polyreactive ligands.

Figure 1.

(A) Polyreactivity ELISA OD405 values of selected mouse IgA mAbs (338E6, 43G10, and 41C10) tested at different concentrations against a polyreactivity panel of diverse antigens: DNA, insulin, lipopolysaccharide (LPS), flagellin, cardiolipin, albumin, and keyhole limpet haemocyanin (KLH). Horizontal dashed line shows cutoff OD405 for positive reactivity.

(B) ELISA OD405 measurements of additional ligands: lysozyme, ubiquitin, and 50-nt ssDNA, against the 43G10, 41C10, and 338E6 mAbs, tested as in (A).

(C) BLI binding plot between immobilized biotinylated 25-nt ssDNA and the Fabs of polyreactive mouse IgA at different concentrations (1 μM–1 mM range). Traces were reference subtracted, and kinetic parameters were calculated by fitting the data globally with Forte bio build-in analysis software (fit curves are shown as gray lines).