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. Author manuscript; available in PMC: 2024 Feb 10.
Published in final edited form as: J Vis Exp. 2022 Feb 8;(180):10.3791/62336. doi: 10.3791/62336

Figure 3: Dynamic time-lapsed expansion of PBNK cells.

Figure 3:

(A) Fold expansion of PBNK during a 22- day time course. Cells were stained with anti-CD56 and anti-CD3 at indicated days for flow cytometry. The total number of NK cells was determined by multiplying NK cell purity to the total number of cells. Expansion rate was generated as follows: (Number of NK cells)Tn/(Number of NK cells)T0, where Number of NK cells = (percentage of NK cell purity) × (total number of cells), T0 is the number of NK cells at time day 0, and Tn is the number of NK cells at time day n. (B) NK cell purity during a 22-day time course. The NK cell expansion was repeated two times with two different donors. Error bars represent ± SEM.