Table of Materials
| Name | Ingredients | Company | Catalog Number | Comments |
|---|---|---|---|---|
| 100 mm surface treated sterile tissue culture dishes | VWR | 10062-880 | For transfection | |
| 293T cells | ATCC | CRL-3216 | For transfection | |
| 6-well G-REX plate | Wilson Wolf | 80240M | For NK cell expansion | |
| AF647-conjugated AffiniPure F(ab’)2 fragment goat anti-human IgG (H+L) | Jackson ImmunoResearch | 109-606-088 | For flow cytometry | |
| CAR construct in SFG vector | Generated in Dr. Dongfang Liu’s lab | For transfection | ||
| Collagenase IV | Sigma | C4-22-1G | For TILs isolation | |
| Cryopreserve media | Fetal Bovine Serum (FBS) | Corning | 35-010-CV | 90% |
| Dimethyl sulfoxide (DMSO) | Sigma | D2050 | 10% | |
| D-10 media | DMEM | VWR | 45000-304 | |
| Fetal Bovine Serum (FBS) | Corning | 35-010-CV | 10% | |
| Penicillin Streptamycin | VWR | 45000-652 | 1% | |
| FastFlow & Low Binding Millipore Express PES Membrane | Millex | SLHPR33RB | For transduction | |
| Genejuice transfection reagent | VWR | 80611-356 | For transfection | |
| gentleMACS C-tubes | Miltenyi Biotec | 130-093-237 | For TILs isolation | |
| gentleMACS Octo | Miltenyi Biotec | Quote | For TILs isolation | |
| Hank’s Balanced Salt Solution (HBSS - w/o calcium or magnesium) | ThermoFisher | 14170120 | For TILs isolation | |
| Human IL-15 | Peprotech | 200-15 | For NK cell expansion | |
| Human IL-2 | Peprotech | 200-02 | For NK cell expansion | |
| Irradiated 221-mIL21 feeder cells | Generated in Dr. Dongfang Liu’s lab | Frozen in cryopreserve media | ||
| Lymphocyte Separation Media | Corning | 25-072-CV | For TILs isolation | |
| OPTI-MEM | ThermoFisher | 31895 | For transfection | |
| PE anti-human CD3 clone HIT3a | Biolegend | 300308 | For flow cytometry | |
| PE/Cy7 anti-human CD56 (NCAM) clone 5.1H11 | BioLegend | 362509 | For flow cytometry | |
| Pegpam3 plasmid | Generated in Dr. Dongfang Liu’s lab | For transfection | ||
| Percoll | GE Healthcare | 17089101 | For TILs isolation | |
| Peripheral blood mononuclear cells (PBMCs) | New York Blood Center | Isolated from plasma of healthy donors, frozen in cryopreserve media | ||
| Phosphate Buffer Saline (PBS) | Corning | 21-040-CV | For transduction | |
| pRDF plasmid | Generated in Dr. Dongfang Liu’s lab | For transfection | ||
| R-10 media | RPMI 1640 | VWR | 45000-404 | |
| L-glutamine | VWR | 45000-304 | 1% | |
| Penicillin Streptamycin | VWR | 45000-652 | 1% | |
| Fetal Bovine Serum (FBS) | Corning | 35-010-CV | 10% | |
| Retronectin | Generated in Dr. Dongfang Liu’s lab | For transduction | ||
| Trypan Blue | Corning | 25-900-Cl | For cell counting | |
| Untreated 24-well plate | Fisher Scientific | 13-690-071 | For transduction |