Table 2.

Mean FRET Efficiencies of NP 4E-BP2 Clusters Compared to Experimental Apo and eIF4E-Bound FRET Values via a z-Test^a

Cluster #	E 32–91	E 73–121	Bound z32–91	Bound z73–121	Apo z32–91	Apo z73–121
Cluster 1	0.34	0.56	2.1	1.4	8.2	0.5
Cluster 2	0.70	0.60	12.2	2.4	1.9	0.5
Cluster 3	0.65	0.62	10.8	3.0	0.6	1.0
Cluster 4	0.58	0.49	9.0	0.6	1.3	2.6

^aExperimental values taken from the literature;²⁶ uncertainties of back-calculated FRET efficiencies are ±0.036 (see Methods).