TABLE 3.
Expression of I-Ad/I-Ed molecules on F4/80+ or CD11b+ spleen cells from normal uninfected mice and from infected mice treated with NRG or anti-IL-12 antibodies
| Mouse group | Mean fluorescence intensity (FL2) of I-Ad/I-Ed ona:
|
|||
|---|---|---|---|---|
| F4/80+ cells
|
CD11b+ cells
|
|||
| Day 7 | Day 13 | Day 7 | Day 13 | |
| Normal | 91 ± 13 | 82 ± 5 | 24 ± 2 | 20 ± 1 |
| NRG treated | 750 ± 50 | 1,163 ± 233 | 421 ± 45 | 615 ± 76 |
| Anti-IL-12 treated | 146 ± 13 | 75 ± 16 | 61 ± 7 | 19 ± 9 |
F4/80+ or CD11b+ cells were gated by using FL1. The results are expressed as the geometric means of fluorescence intensity (FL2). The data represent the means ± standard deviations of three similar experiments. The geometric mean of fluorescence intensity when staining with the conjugated isotype control antibodies was less than 10 for all three groups of mice.