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. 2024 Feb 10;73(2):40. doi: 10.1007/s00262-023-03582-5

Table 3.

Expression levels of proteins and phosphorylation level of Drp1 and ERK in the co-cultured groups (mean ± SD)

Group Drp1 ERK1/2 p-Drp1S616 p-ERK1/2T202Y204
wtT 3.67 ± 2.08 4.00 ± 1.00 4.33 ± 1.53 2.67 ± 0.58
wtT + PD-1 mAb 2.67 ± 0.58 6.33 ± 4.93 5.33 ± 1.15 5.67 ± 0.58
shDT + PD-1 mAb 3.00 ± 1.00 2.67 ± 0.58 4.67 ± 1.53 4.33 ± 2.08
oeDT + PD-1 mAb 4.33 ± 3.21 2.67 ± 0.58 8.33 ± 3.21 6.67 ± 2.08
wtT + A549 3.00 ± 1.00a 3.00 ± 1.00 5.33 ± 2.31a 3.67 ± 1.15a
shNT + A549 4.67 ± 3.79 2.33 ± 0.58 5.33 ± 1.53 4.67 ± 1.53
shDT + A549 3.67 ± 1.15 3.67 ± 0.58 4.33 ± 2.08 3.67 ± 2.08
oeNT + A549 3.67 ± 1.15 3.00 ± 1.00 4.67 ± 1.53 4.67 ± 0.58
oeDT + A549 4.67 ± 1.53 2.67 ± 0.58 5.00 ± 1.00b 5.67 ± 0.58b
wtT + PBS + A549 8.33 ± 2.08 3.00 ± 1.00 5.33 ± 3.21 4.67 ± 1.53
wtT + PD-1 mAb + A549 4.00 ± 1.73 3.00 ± 1.00 6.33 ± 1.53 7.33 ± 1.15c
shDT + PD-1 mAb + A549 10.00 ± 5.29 3.33 ± 1.53 5.33 ± 1.53c 4.67 ± 1.53d
oeDT + PD-1 mAb + A549 7.33 ± 3.79a 5.33 ± 2.52 9.67 ± 3.21abc 13.33 ± 1.53abcd

The same superscript letter (e.g., a, b, c) indicates a significant difference between the groups (p < 0.05). Abbreviations: wtT: wild-type CD3+ T cells; shDT: CD3+ T cells with Drp1 knockdown; oeDT: CD3+ T cells with Drp1 overexpression; shNT and oeNT: negative control groups of T-shDrp1 and T-oeDrp1, respectively; PD-1 mAb: programmed cell death protein 1 monoclonal antibody; PBS: phosphate-buffered saline