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. 2024 Feb 10;15:1256. doi: 10.1038/s41467-024-45451-4

Fig. 6. The key amino acids mediating LLPS of LIN28A is required for its role in facilitating reprogramming of mouse embryonic fibroblast cells(MEF).

Fig. 6

a Reprogramming efficiency of MEF cells transduced with OCT4, SOX2, NANOG, and LIN28A variants. b Number of iPSC colonies 14 days after OSNA transduction of MEF cells. n = 3 biologically independent experiments. One-way ANOVA. Data are presented as mean values +/− SEM. c Representative STED immunofluorescence images of LIN28A and NPM in MEF or iPSCs. NPM applied STED pattern. d Representative STED immunofluorescence images of NPM and FBL in MEF or iPSCs. FBL applied STED pattern. e Statistical analysis of the GC(NPM) irregularity in MEF and iPSCs using Boyce-Clark semidiameter index. The larger the number, the more irregular the NPM. n = 20 nucleoli, unpaired two-tailed student’s t-test. f Graph showing the number of typical DFC(FBL) morphology in MEF or iPSCs. n = 20 nucleoli. Fisher Exact Test, two-sided; MEF vs iPSC:p = 0.000244362. g A cartoon diagram showing morphological changes of NPM and FBL in MEF and iPSCs. h Images and OP-Puro intensity statistical analysis of OP-puromycin-labeled MEF and iPSCs. Scale bar, 20 µm. n = 20 cells, unpaired two-tailed student’s t-test. The center line is the median, the bottom of the box is the 25th percentile boundary, the top of the box is the 75th percentile, and the top and bottom of the vertical line define the boundary of the data. Source data are provided as a Source Data file.