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. 2024 Feb 10;15:1274. doi: 10.1038/s41467-024-45386-w

Fig. 2. dTAG-Matr3 could be degraded within 4 h., and acute depletion of Matr3 contributes to few changes in nascent RNA production.

Fig. 2

a Strategy to knockin FKBP F36v at the 5’ end of Matr3. The dTAG-47 PROTAC recruits the CRBN E3 ligase complex to FKBP F36v-Matr3. b High-efficient knockin bulk FKBP F36v-Matr3 were sorted by GFP and confirmed by Western blot. Cells enriched in the top 0.2% highest GFP signal were sorted (GFP-sorted). FKBP F36v-Matr3 (refer as dTAG-Matr3) bands were enriched in the GFP-sorted bulk, and tagging efficiency was also confirmed by HA antibody. The confirmation of Knockin was repeated 3 times independently with similar results. c Matr3 protein was depleted within 4 h. upon dTAG47 exposure. Western blots of dTAG-Matr3 upon dTAG47 (500 nM) treatment in a time course. Confirmation of degradation was repeated 3 times independently with similar results. d Nascent RNA and total RNA were quantified by SLAM-seq upon 4 h. Matr3 depletion. In volcano plots, p values from the Wald test less than 0.05 (p < 0.05) for red line, and log2FC > 0.4 for the vertical blue lines denote significant DE genes. Source data are provided as a Source Data file.