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. 2024 Feb 10;15:1274. doi: 10.1038/s41467-024-45386-w

Fig. 5. Loss of Matr3 disrupts YY1 binding and YY1-enriched cohesin loading.

Fig. 5

a YY1 binding enhanced upon Matr3 depletion (4 h.). Volcano plot showing the logFoldChange vs. -log P value of YY1 binding changes (measured by ∆=dTAG-DMSO treatment). Red line: significance line (P = 0.05). Wald test are corrected for multiple comparison using the BH method. b Co-occurrence of differential YY1 occupancy with MyoD, CTCF, Rad21, ATAC-seq. Number of shared differential peaks between YY1 increased (4 h.) and the gained/lost portion (4 h.) of MyoD, CTCF, Rad21, or ATAC-seq. Box: the overlap percentage with Rad21 binding loss was highest, showing a relationship between YY1 differential binding and cohesin loss. c Heatmap showing that Rad21 binding was lost at sites of YY1 binding increase. Dashed line on the histogram shows the reference line to aid comparison between dTAG47 treatment and DMSO. d Heatmap showing MyoD binding was enhanced at sites of YY1 binding increase. e Heatmap showing ATAC-seq peaks were decreased at sites of YY1 binding increase. Source data are provided as a Source Data file.