Fig. 8. Matr3 mediates differentiation through stabilizing chromatin accessibility and chromatin loop-domain interactions, and YY1 mediated enhancer-promoter interactions.

Acute depletion of Matr3 loss immediately reduced cohesin loading, and destabilized chromatin structural loops and affected long distance interactions. As a mechanism for partial compensation of loop re-arrangement, YY1 was recruited to sites of cohesin loss, as well as enhancers and promoter sites, and formed new E-P loops, thereby disrupting the E-P loop landscape. During myogenesis, MyoD was recruited to those chromatin sites, leading to gene expression changes as skeletal muscle development progressed.