Fig. 2.

Heatstroke induces neuron apoptosis. Heatstroke rat model was prepared with a Tc that reached 41 °C with subsequent cooling at ambient temperature for 2 h, 6 h, and 24 h (A & B) TUNEL was used to detect the apoptosis of cerebral cortex and hippocampus. (C) Corresponding quantifications of TUNEL positive cells were calculated and are shown. ∗p < 0.05 vs. control group. (D & E) Active caspase-3 expression was detected by immunohistochemistry in cerebral cortex and hippocampus. Corresponding quantifications of positive cells were calculated and are shown in F. ∗p < 0.05 vs. control group. ∗p < 0.05 vs. control group, #p < 0.05 vs. control group. (G) Expression level of active caspase-3 was detected by western blot. Left: diagram of gel electrophoresis. Right: statistical graph. ∗p < 0.05 vs. control group.

Tc: core temperature; TUNEL: triphosphate gap terminal labeling; IPI: immunohistochemical positive index.