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. 2024 Feb 12;15:1280. doi: 10.1038/s41467-024-45520-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a BMDMs were treated with 400 pg/μL Shh for 24 h, the expression of inflammatory genes was analyzed by qRT-PCR. n = 4 independent experiments per group. b Relative exosome amount was analyzed in the liver of Hsp90β^fl/fl or Hsp90β^ΔHep mice fed with chow or HFFC diet. (n = 3 mice per group, chow+Hsp90β^fl/fl versus HFFC+Hsp90β^fl/fl, P = 0.027, HFFC +Hsp90β^fl/fl versus HFFC+ Hsp90β^ΔHep, P = 0.0046). c BMDMs were treated with exosomes derived from livers in Hsp90β^fl/fl and Hsp90β^ΔHep mice fed with chow or HFFC for 24 h, the expression of inflammatory genes was analyzed by qRT-PCR (n = 3 mice per group, for Il-1β, chow+Hsp90β^fl/fl versus HFFC+Hsp90β^fl/fl, P = 9.7E-06, HFFC +Hsp90β^fl/fl versus HFFC+ Hsp90β^ΔHep, P = 1.8E-05; for Tnf-α, chow+Hsp90β^fl/fl versus HFFC+Hsp90β^fl/fl, P = 4.8E-08, HFFC +Hsp90β^fl/fl versus HFFC+ Hsp90β^ΔHep, P = 1.0E-07; for Il-6, chow+Hsp90β^fl/fl versus HFFC+Hsp90β^fl/fl, P = 1.0E-05, HFFC +Hsp90β^fl/fl versus HFFC+ Hsp90β^ΔHep, P = 1.7E-05). d Exosomes were collected from livers of Hsp90β^fl/fl and Hsp90β^ΔHep mice fed with chow or HFFC diet, the expression of exosomes containing miRNA were analyzed by sequencing, the heatmap of miRNA expression was shown. c represented Hsp90β^fl/fl mice on a normal chow diet. M represented Hsp90β^fl/fl mice on a HFFC diet. KO represented Hsp90β^ΔHep mice on a HFFC diet. n = 3 mice per group. e BMDM cells were administrated with indicated miRNAs for 24 h, the expression of inflammatory genes was analyzed by qRT-PCR (n = 4 independent experiments per group, for Il-1β, miR-NC versus miR-28-5p, P = 0.0031, versus miR-489-3p, P = 0.99, versus miR-500-3p, P = 0.93, versus miR-3473a, P = 0.0029; for Tnf-α, miR-NC versus miR-28-5p, P = 4.3E-07, versus miR-489-3p, P = 0.42, versus miR-500-3p, P = 0.33, versus miR-3473a, P = 3.5E-04; for Il-6, miR-NC versus miR-28-5p, P = 0.0011, versus miR-489-3p, P = 4.1E-08, versus miR-500-3p, P = 0.84, versus miR-3473a, P = 0.0017). f Exosomes were collected from primary hepatocytes of Hsp90β^fl/fl and Hsp90β^ΔHep mice after the treatment with 400 pg/μL Shh for 48 h, and relative expression of miR-28-5p was analyzed by qRT-PCR (n = 3 mice per group, control+Hsp90β^fl/fl versus Shh+Hsp90β^fl/fl, P = 0.0061, Shh+Hsp90β^fl/fl versus Shh+Hsp90β^ΔHep, P = 0.0029, control+Hsp90β^ΔHep versus Shh+Hsp90β^ΔHep, P = 0.60). g Exosomes were collected from sera of healthy donors or NASH patients, and the relative exosome amount was analyzed (n = 19 participants in normal group. n = 9 participants in NASH group, normal versus NASH, P = 0.0022). h Exosomes were collected from sera of healthy donors or NASH patients, and relative expression of miR-28-5p was analyzed by qRT-PCR (n = 19 participants in normal group. n = 9 participants in NASH group, normal versus NASH, P = 2.4E-05). i Serum SHH concentration of healthy donors or NASH patients (n = 19 participants in normal group. n = 9 participants in NASH group, P = 0.19). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, NS no significant difference, one-way ANOVA. Source data are provided in the Source Data file.