FIG. 1.
Modification of the synthetic gene encoding BoNT/A(Hc). (A) Gene construct as described by Clayton et al. (6). (B) Modified gene construct for the work presented in this report. In both sequences, only the regions modified are shown. The cloning restriction sites are shown above the sequence. The initiation and termination codons are shown in bold, and the one-letter amino acid codes are depicted below the gene sequences. The initiation codon represents amino acid 861 of the full-length BoNT/A sequence.