FIG. 2.
Purification of BoNT/A(Hc) by sequential chromatography. (A) Mono S cation-exchange chromatography of the Streamline pool. Proteins were eluted with an increasing NaCl gradient, yielding two immunoreactive peaks. Peak 1 (B) and peak 2 (C) were pooled individually and subjected to HIC, and proteins were eluted with a decreasing ammonium sulfate gradient. Protein production was monitored by A280 (left axis); elution conditions are recorded on the right axis, with a gradient trace laid over the chromatogram.