Table 1.

N-glycan binding lectin properties in the brain. Abbreviations: PNGase F—Peptide:N-glycosidase F; ML—molecular layer; GL—granular layer; AV—arbor vitae; WMT—white matter tracks.

Lectin	PNGase F sensitivity	Competing sugar (200 mM)	Regional Binding Pattern*			Other notable structures
			i	ii	iii
ConA	+	Mannose, Glucose	Diffuse in ML/GL, less in AV	Diffuse, less in WMT	Diffuse	Bright punctate structures, including around Purkinje cell body
GNL	+	Mannose, Glucose	Diffuse in ML/GL, less in AV	Diffuse, less in WMT	Diffuse with increased signal in glomerular layer	Bright punctate structures, including around Purkinje cell body
AAL	+	L-Fucose	Increased in ML, scattered in GL, decreased in AV	Diffuse, less in WMT	Diffuse	−
PHA-E	+	GalNAc, Lactose	Increased in ML, scattered in GL, decreased in AV	Diffuse	Diffuse	Bright signal in choroid plexus and moderate signal in microvasculature
RCA-I	+	Lactose, Galactose	Speckled throughout	Speckled throughout	Speckled with moderate signal in glomerular layer	Bright signal in choroid plexus and microvasculature
SNA	Partial	Lactose	Specific binding in ML and AV, non-specific in GL	Diffuse	Diffuse	Bright signal in choroid plexus
MAL-I	+	Lactose	Increased in ML, scattered in GL, decreased in AV	Diffuse, increased signal near surface	Diffuse, increased signal near surface	−

^*Binding pattern in coronal sections according to Supplementary Fig. 1.