FIG. 5.

Effects of flurofamide (A) and acetohydroxamic acid (B) on the chemotaxis of CPY3401 toward sodium bicarbonate in PVP-chemotaxis buffer. Cells of CPY3401 suspended in PVP-chemotaxis buffer were incubated in the absence (▵) or presence of 1 (○) and 10 (□) μM urease inhibitor at 28°C for 10 min, and the chemotactic activities in response to 10 mM NaHCO₃ were determined. In panel B, accumulation of cells incubated with 100 μM acetohydroxamic acid prior to the chemotaxis assay (•) is also shown. In panel A, bacterial accumulation in PVP-chemotaxis buffer without attractants (■) is also shown. Error bars show standard deviations.