Figure 2.

ALDH2 dysfunction cooperates with alcohol in CD44H cell enrichment by promoting CD44L cell apoptosis. Cells dissociated from IEN MDOs from the indicated genotype were grown in monolayer culture, treated with 2% EtOH for 72 h, collected and assessed for apoptosis by flow cytometry for Annexin V/PI. (A) representative dot plot of Annexin V and PI staining, (B) quantification of (A). (C) Apoptosis was measured in CD44H and CD44L cells by flow cytometry. *P < 0.05 compared with Aldh2*1 controls (B) or with Aldh2*1 CD44L cells (C) #P < 0.05 compared with Aldh2*2 control (A) or with Aldh2*2 CD44L cells (C). Circles represent technical replicates (n = 3).