FIG. 1.
Effect of antimannan IgG on the kinetics for C3 deposition on C. albicans yeast cells via the alternative pathway. Yeast cells were incubated in a C3 binding medium containing 40% NHS, 40% EGTA-chelated NHS, 40% mannan-absorbed and EGTA-chelated NHS, or 40% mannan-absorbed and EGTA-chelated NHS supplemented with antimannan IgG at 88 μg per ml of reaction mixture, which was 40% of antimannan IgG concentration found in the pooled serum used for this experiment.