FIG. 3.

Dose effect of exogenous antimannan IgG on binding of C3 to C. albicans via the classical or alternative pathway. C3 binding reactions were supplemented with various amounts of antimannan IgG per milliliter of binding mixture that contained either 40% mannan-absorbed serum to assess the ability of yeast cells to activate the classical pathway following a 6-min incubation or 40% mannan-absorbed and EGTA-chelated serum to assess the ability of yeast cells to activate the alternative pathway following a 12 min incubation.