Table 2:
Usage of monoclonal antibodies.
| Antigen | Clone | Fluorochrome | Dilution | Surface/intracellular |
|---|---|---|---|---|
| CD45 | 30-F11 | APC/CY7 | 1:100 | surface |
| Gr-1 | RB6-8C5 | BV421 | 1:100 | surface |
| CD68 | FA-11 | PerCPCy5.5 | 1:100 | intracellular |
| CD11b | M1/70 | PECy7 | 1:100 | surface |
| Siglec F | S17007L | FITC | 1:100 | surface |
| CD11c | N418 | BV650 or BV510 | 1:100 | surface |
| CD64 | X54-5/7.1 | PE/Dazzle 594 | 1:100 | surface |
| MHC-II | M5/114.15.2 | AF700 | 1:100 | surface |
| CD103 | 2.00E+07 | PE / FITC | 1:100 | surface |
| Live/Dead Fixable Far Read Dead Cell Stain Kit |
FarRed (APC) or Aqua (BV510) |
1:1000 | surface | |
| CD3 | 17A2 | PE | 1:100 | surface |
| B220 | RA3-6B2 | AF488 | 1:100 | surface |
| NK1.1 | PK136 | FITC | 1:100 | surface |
| CD24 | 30-F1 | PE | 1:100 | surface |
| MERTK | 2B10C42 | PE | 1:100 | surface |
| F4/80 | BM8 | BV605 | 1:100 | surface |
| CX3CR1 | SA011F11 | PE | 1:100 | surface |
| FcBlock (CD16/32) | 93 | 1:100 | surface |