TABLE 1.
Immunization regimen | IFN-γ concn (U/ml) | IL-2 concn (U/ml) | IL-5 concn (U/ml) |
---|---|---|---|
DC∼TSo | 1,050 | 0.70 | <0.45 |
DC | <0.9 | <0.08 | <0.45 |
TSo | <0.9 | <0.08 | <0.45 |
Mice were injected in the footpads with TSo-pulsed DC (DC∼TSo) or unpulsed DC (DC) or with TSo alone (TSo) or were untreated. Lymph node cells recovered 5 days after immunization were cultured with TSo. The culture supernatants were examined for cytokine production. The peak levels of cytokine in the supernatant are given. Values for IL-2, IFN-γ, and IL-5 were measured at 24, 72, and 96 h of culture, respectively. The results are representative of at least three experiments.