FIG. 2.

B. burgdorferi does not affect the rate at which monocytes exit from HUVEC-amnion cultures. HUVEC-amnion cultures were either left unstimulated or pretreated for 8 h with B. burgdorferi N40 at a ratio of 100 Bb/EC. Monocytes were then added to the cultures for 2 h. Cultures were washed and either fixed immediately or incubated for an additional 22 or 96 h. The number of monocytes remaining in the amniotic tissue was then determined as described in Materials and Methods. Data are presented as the percentage of monocytes that remained in the amniotic tissue relative to the number that had migrated after 2 h. Bars represent the means ± SD of four to five replicate samples. This experiment was repeated twice with similar results.